Open discussion platform of the COST action EuroMicropH. This discussion series is intended to stimulate an exchange on the different aspects of how microorganisms react to low pH conditions and why people are interested to investigate this subject.
17.02.2023 15:00 CET
Zoom
https://tuwien.zoom.us/j/99083643951?pwd=MXA1THg3cWw1WWxyZ1NGRzEwekxRdz09
Meeting-ID: 990 8364 3951
Password: Ln1gCBTY
Agenda
15:00 Wellcome by the discussion leaders Carmit Ziv and Ott Scheler
15:05 Moshe Shemesh, Department of Food Science, ARO, Volcani Institute, Israel
Enhancing physiological persistence of probiotic bacteria through adaptive geometrical structuring in acidic pH.
15:30 Pirjo Spuul, Department of Chemistry and Biotechnology , Tallinn University of Technology, Estonia
Isolation and characterization of novel Lactobacillus salivarius strain from gastric biopsy.
Abstracts
Enhancing physiological persistence of probiotic bacteria through adaptive geometrical structuring in acidic pH.
Moshe Shemesh
The lactic acid bacteria (LAB), such as Lactiplantibacillus plantarum can positively modulate different aspects of human health. Nonetheless, they need to be physiologically functionalized to insure their survivability during the passage through the upper gastrointestinal tract (GIT). Our results indicate that cells of L. plantarum tend to form complex geometrical structures during their growth in an acidic pH environment. We further find that this geometrical structuring represents adaptive developmental phase prior transition to protective – biofilm mode of life, which supports the survivability and persistence of LAB in acidic and other stress environments. We therefore believe that inducing physiological persistence of LAB, using acid adapting growth system, enables enhancing their survivability and probiotic functionality within various hostile conditions.
Isolation and characterization of novel Lactobacillus salivarius strain from gastric biopsy.
Pirjo Spuul
Lactobacillus salivarius (L. salivarius) is one of the few bacterial species capable of surviving in the hostile environment of the human stomach. Probiotic Lactobacilli, including L. salivarius, have been suggested to be useful for the eradication of the human pathogen Helicobacter pylori (H. pylori). We have isolated novel L. salivariusstrain while cultivating H. pylori on Columbia blood agar (CBA) supplemented with vancomycin, trimethoprim, cefsulodin, and amphotericin B from a stomach biopsy sample. The new strain has been characterized, its pH tolerance tested and most importantly, its effect on H. pylori analysed. Interestingly, L. salivarius strain A1 suppresses but does not entirely inhibit the growth of H. pylori. In addition, the presence of A1 reduces the inflammatory response of H. pylori.
