Training School: Imaging microbial response to low pH

Training School: Imaging microbial response to low pH
Date: 22 May 2023 - 26 May 2023
Venue: Collège de France, Paris, France
Location: Paris, France

We are pleased to announce that the Center for Interdisciplinary Research in Biology at the Collège de France in Paris is organizing a training school (TS) on microscopy entitled “Imaging microbial response to low pH”.

The Training School will address the theory and practical application of the basics of observing microorganism populations. The theoretical part will consist of a series of lectures by experts in the field. 

The practical part will include:

– preparation of bacteria with the appropriate biosensors for pH stress

– infection of macrophages with the bacteria with biosensor

– immunostaining of the infected macrophage revealing acidification of the bacterial compartment

– imaging of the bacteria grown in the bacterial compartment

– expression of the biosensor in the whole population (fluorimetry)

– introduction to the basic functions of image J

– image analysis with a custom image J plugin for bacteria (microbe J)

Trainers

 Romain Briandet Micalis INRAE Paris-Saclay University; Manuel Campos LMGM Toulouse University; Adrien Ducret MMBS CNRS Lyon I University; Tristan Piolot Collège de France, PSL Paris ; Local organisers: Sylvie Rimsky and Olivier Espéli

Applicants

The TS is designed for beginners: PhD students or early-career Postdoc

Application procedure and deadline

If you are interested in applying for this Training School, please fill out this form.

Deadline for applications

28th February 2023

If you need more information, please write to the organizers .

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